Reference documentation

Documentation for module functions (for developers)

bgmm.py

Functions used to fit the mixture model to a database. Access using BGMMFit.

BGMM using sklearn

PopPUNK.bgmm.assign_samples(X, weights, means, covars, scale, values=False)

Given distances and a fit will calculate responsibilities and return most likely cluster assignment

Args:

X (numpy.array)

n x 2 array of core and accessory distances for n samples

weights (numpy.array)

Component weights from BGMMFit

means (numpy.array)

Component means from BGMMFit

covars (numpy.array)

Component covariances from BGMMFit

scale (numpy.array)

Scaling of core and accessory distances from BGMMFit

values (bool)

Whether to return the responsibilities, rather than the most likely assignment (used for entropy calculation).

Default is False

Returns:

ret_vec (numpy.array)

An n-vector with the most likely cluster memberships or an n by k matrix with the component responsibilities for each sample.

PopPUNK.bgmm.findWithinLabel(means, assignments, rank=0)

Identify within-strain links

Finds the component with mean closest to the origin and also akes sure some samples are assigned to it (in the case of small weighted components with a Dirichlet prior some components are unused)

Args:

means (numpy.array)

K x 2 array of mixture component means

assignments (numpy.array)

Sample cluster assignments

rank (int)

Which label to find, ordered by distance from origin. 0-indexed.

(default = 0)

Returns:

within_label (int)

The cluster label for the within-strain assignments

PopPUNK.bgmm.fit2dMultiGaussian(X, dpgmm_max_K=2)

Main function to fit BGMM model, called from fit()

Fits the mixture model specified, saves model parameters to a file, and assigns the samples to a component. Write fit summary stats to STDERR.

Args:

X (np.array)

n x 2 array of core and accessory distances for n samples. This should be subsampled to 100000 samples.

dpgmm_max_K (int)

Maximum number of components to use with the EM fit. (default = 2)

Returns:

dpgmm (sklearn.mixture.BayesianGaussianMixture)

Fitted bgmm model

PopPUNK.bgmm.log_likelihood(X, weights, means, covars, scale)

modified sklearn GMM function predicting distribution membership

Returns the mixture LL for points X. Used by assign_samples() and plot_contours()

Args:

X (numpy.array)

n x 2 array of core and accessory distances for n samples

weights (numpy.array)

Component weights from fit2dMultiGaussian()

means (numpy.array)

Component means from fit2dMultiGaussian()

covars (numpy.array)

Component covariances from fit2dMultiGaussian()

scale (numpy.array)

Scaling of core and accessory distances from fit2dMultiGaussian()

Returns:

logprob (numpy.array)

The log of the probabilities under the mixture model

lpr (numpy.array)

The components of the log probability from each mixture component

PopPUNK.bgmm.log_multivariate_normal_density(X, means, covars, min_covar=1e-07)

Log likelihood of multivariate normal density distribution

Used to calculate per component Gaussian likelihood in assign_samples()

Args:

X (numpy.array)

n x 2 array of core and accessory distances for n samples

means (numpy.array)

Component means from fit2dMultiGaussian()

covars (numpy.array)

Component covariances from fit2dMultiGaussian()

min_covar (float)

Minimum covariance, added when Choleksy decomposition fails due to too few observations (default = 1.e-7)

Returns:

log_prob (numpy.array)

An n-vector with the log-likelihoods for each sample being in this component

dbscan.py

Functions used to fit DBSCAN to a database. Access using DBSCANFit.

DBSCAN using hdbscan

PopPUNK.dbscan.assign_samples_dbscan(X, hdb, scale)

Use a fitted dbscan model to assign new samples to a cluster

Args:

X (numpy.array)

N x 2 array of core and accessory distances

hdb (hdbscan.HDBSCAN)

Fitted DBSCAN from hdbscan package

scale (numpy.array)

Scale factor of model object

Returns:

y (numpy.array)

Cluster assignments by sample

PopPUNK.dbscan.evaluate_dbscan_clusters(model)

Evaluate whether fitted dbscan model contains non-overlapping clusters

Args:

model (DBSCANFit)

Fitted model from fit()

Returns:

indistinct (bool)

Boolean indicating whether putative within- and between-strain clusters of points overlap

PopPUNK.dbscan.findBetweenLabel(assignments, within_cluster)

Identify between-strain links from a DBSCAN model

Finds the component containing the largest number of between-strain links, excluding the cluster identified as containing within-strain links.

Args:

assignments (numpy.array)

Sample cluster assignments

within_cluster (int)

Cluster ID assigned to within-strain assignments, from findWithinLabel()

Returns:

between_cluster (int)

The cluster label for the between-strain assignments

PopPUNK.dbscan.fitDbScan(X, min_samples, min_cluster_size, cache_out)

Function to fit DBSCAN model as an alternative to the Gaussian

Fits the DBSCAN model to the distances using hdbscan

Args:

X (np.array)

n x 2 array of core and accessory distances for n samples

min_samples (int)

Parameter for DBSCAN clustering ‘conservativeness’

min_cluster_size (int)

Minimum number of points in a cluster for HDBSCAN

cache_out (str)

Prefix for DBSCAN cache used for refitting

Returns:

hdb (hdbscan.HDBSCAN)

Fitted HDBSCAN to subsampled data

labels (list)

Cluster assignments of each sample

n_clusters (int)

Number of clusters used

mash.py

Mash functions for database construction

PopPUNK.mash.checkMashVersion(mash_exec)

Checks that mash can be run, and is version 2 or higher. Exits if version < 2.

Args:

mash_exec (str)

Location of mash executable

Returns:

version (str)

Version string

PopPUNK.mash.constructDatabase(assemblyList, klist, sketch_size, oPrefix, ignoreLengthOutliers=False, threads=1, overwrite=False, reads=False, mash_exec='mash')

Sketch the input assemblies at the requested k-mer lengths

A multithread wrapper around runSketch(). Threads are used to either run multiple sketch processes for each klist value, or increase the threads used by each mash sketch process if len(klist) > threads.

Also calculates random match probability based on length of first genome in assemblyList.

Args:

assemblyList (str)

File with locations of assembly files to be sketched

klist (list)

List of k-mer sizes to sketch

sketch_size (int)

Size of sketch (-s option)

oPrefix (str)

Output prefix for resulting sketch files

ignoreLengthOutliers (bool)

Whether to check for outlying genome lengths (and error if found)

(default = False)

threads (int)

Number of threads to use

(default = 1)

overwrite (bool)

Whether to overwrite sketch DBs, if they already exist.

(default = False)

reads (bool)

If reads are being used as input

(default = False)

mash_exec (str)

Location of mash executable

(default = ‘mash’)

PopPUNK.mash.createDatabaseDir(outPrefix, kmers)

Creates the directory to write mash sketches to, removing old files if unnecessary

Args:

outPrefix (str)

output db prefix

kmers (list)

k-mer sizes in db

PopPUNK.mash.fitKmerBlock(idxRanges, distMat, raw, klist, jacobian)

Multirow wrapper around fitKmerCurve() to the specified rows in idxRanges

Args:

idxRanges (int, int)

Tuple of first and last row of slice to calculate

distMat (numpy.array)

sharedmem object to store core and accessory distances in (altered in place)

raw (numpy.array)

sharedmem object with proportion of k-mer matches for each query-ref pair by row, columns are at k-mer lengths in klist

klist (list)

List of k-mer lengths to use

jacobian (numpy.array)

The Jacobian for the fit, sent to fitKmerCurve()

PopPUNK.mash.fitKmerCurve(pairwise, klist, jacobian)

Fit the function \(pr = (1-a)(1-c)^k\)

Supply jacobian = -np.hstack((np.ones((klist.shape[0], 1)), klist.reshape(-1, 1)))

Args:

pairwise (numpy.array)

Proportion of shared k-mers at k-mer values in klist

klist (list)

k-mer sizes used

jacobian (numpy.array)

Should be set as above (set once to try and save memory)

Returns:

transformed_params (numpy.array)

Column with core and accessory distance

PopPUNK.mash.getDatabaseName(prefix, k)

Gets the name for the mash database for a given k size

Args:

prefix (str)

db prefix

k (str)

k-mer size

Returns:

db_name (str)

Name of mash db

PopPUNK.mash.getKmersFromReferenceDatabase(dbPrefix)

Get kmers lengths from existing database

Parses the database name to determine klist

Args:

dbPrefix (str)

Prefix for sketch DB files

Returns:

kmers (list)

List of k-mer lengths used in database

PopPUNK.mash.getSeqsInDb(mashSketch, mash_exec='mash')

Return an array with the sequences in the passed mash database

Calls mash info -t

Args:

mashSketch (str)

Mash sketches/database

mash_exec (str)

Location of mash executable

Returns:

seqs (list)

List of sequence names in sketch DB

PopPUNK.mash.getSketchSize(dbPrefix, klist, mash_exec='mash')

Call to mash info to determine sketch size

sys.exit(1) is called if DBs have different sketch sizes

Args:

dbprefix (str)

Prefix for mash databases

klist (list)

List of k-mer lengths which databases were constructed at

mash_exec (str)

Location of mash executable

Returns:

sketchdb (dict)

Dict of sketch sizes indexed by k-mer size

PopPUNK.mash.init_lock(l)

Sets a global lock to use when writing to STDERR in runSketch()

PopPUNK.mash.joinDBs(db1, db2, output, klist, mash_exec='mash')

Join two mash sketch databases with mash paste

Args:

db1 (str)

Prefix for db1

db2 (str)

Prefix for db2

output (str)

Prefix for joined output

klist (list)

List of k-mer sizes to sketch

mash_exec (str)

Location of mash executable

(default = ‘mash’)

PopPUNK.mash.queryDatabase(rNames, qNames, dbPrefix, queryPrefix, klist, self=True, number_plot_fits=0, no_stream=False, mash_exec='mash', threads=1)

Calculate core and accessory distances between query sequences and a sketched database

For a reference database, runs the query against itself to find all pairwise core and accessory distances.

Uses the relation \(pr(a, b) = (1-a)(1-c)^k\)

To get the ref and query name for each row of the returned distances, call to the iterator iterDistRows() with the returned refList and queryList

Args:

rNames (list)

Names of ref sequences (ignored)

qNames (list)

Names of query sequences

dbPrefix (str)

Prefix for reference mash sketch database created by constructDatabase()

queryPrefix (str)

Prefix for query mash sketch database created by constructDatabase()

klist (list)

K-mer sizes to use in the calculation

self (bool)

Set true if query = ref

(default = True)

number_plot_fits (int)

If > 0, the number of k-mer length fits to plot (saved as pdfs). Takes random pairs of comparisons and calls plot_fit()

(default = 0)

no_stream (bool)

Rather than streaming mash dist input directly into parser, will write through an intermediate temporary file

(default = False)

mash_exec (str)

Location of mash executable

(default = ‘mash’)

threads (int)

Number of threads to use in the mash process

(default = 1)

Returns:

refList (list)

Names of reference sequences

queryList (list)

Names of query sequences

distMat (numpy.array)

Core distances (column 0) and accessory distances (column 1) between refList and queryList

PopPUNK.mash.readMashDBParams(dbPrefix, kmers, sketch_sizes, mash_exec='mash')

Get kmers lengths and sketch sizes from existing database

Calls getKmersFromReferenceDatabase() and getSketchSize() Uses passed values if db missing

Args:

dbPrefix (str)

Prefix for sketch DB files

kmers (list)

Kmers to use if db not found

sketch_sizes (list)

Sketch size to use if db not found

mash_exec (str)

Location of mash executable

Default = ‘mash’

Returns:

kmers (list)

List of k-mer lengths used in database

sketch_sizes (list)

List of sketch sizes used in database

PopPUNK.mash.runSketch(k, assemblyList, sketch, genome_length, oPrefix, mash_exec='mash', overwrite=False, threads=1)

Actually run the mash sketch command

Called by constructDatabase()

Args:

k (int)

k-mer size to sketch

assemblyList (list)

Locations of assembly files to be sketched

sketch (int)

Size of sketch (-s option)

genome_length (int)

Length of genomes being sketch, for random match probability calculation

oPrefix (str)

Output prefix for resulting sketch files

mash_exec (str)

Location of mash executable

(default = ‘mash’)

overwrite (bool)

Whether to overwrite sketch DB, if it already exists.

(default = False)

threads (int)

Number of threads to use in the mash process

(default = 1)

models.py

Classes used for model fits

class PopPUNK.models.BGMMFit(outPrefix, max_samples=100000)

Class for fits using the Gaussian mixture model. Inherits from ClusterFit.

Must first run either fit() or load() before calling other functions

Args:

outPrefix (str)

The output prefix used for reading/writing

max_samples (int)

The number of subsamples to fit the model to

(default = 100000)

assign(X, values=False)

Assign the clustering of new samples using assign_samples()

Args:

X (numpy.array)

Core and accessory distances

values (bool)

Return the responsibilities of assignment rather than most likely cluster

Returns:

y (numpy.array)

Cluster assignments or values by samples

fit(X, max_components)

Extends fit()

Fits the BGMM and returns assignments by calling fit2dMultiGaussian().

Fitted parameters are stored in the object.

Args:

X (numpy.array)

The core and accessory distances to cluster. Must be set if preprocess is set.

max_components (int)

Maximum number of mixture components to use.

Returns:

y (numpy.array)

Cluster assignments of samples in X

load(fit_npz, fit_obj)

Load the model from disk. Called from loadClusterFit()

Args:

fit_npz (dict)

Fit npz opened with numpy.load()

fit_obj (sklearn.mixture.BayesianGaussianMixture)

The saved fit object

plot(X, y)

Extends plot()

Write a summary of the fit, and plot the results using PopPUNK.plot.plot_results() and PopPUNK.plot.plot_contours()

Args:

X (numpy.array)

Core and accessory distances

y (numpy.array)

Cluster assignments from assign()

save()

Save the model to disk, as an npz and pkl (using outPrefix).

class PopPUNK.models.ClusterFit(outPrefix)

Parent class for all models used to cluster distances

Args:

outPrefix (str)

The output prefix used for reading/writing

fit(X=None)

Initial steps for all fit functions.

Creates output directory. If preprocess is set then subsamples passed X and draws a scatter plot from result using plot_scatter().

Args:

X (numpy.array)

The core and accessory distances to cluster. Must be set if preprocess is set.

(default = None)

no_scale()

Turn off scaling (useful for refine, where optimization is done in the scaled space).

plot(X=None)

Initial steps for all plot functions.

Ensures model has been fitted.

Args:

X (numpy.array)

The core and accessory distances to subsample.

(default = None)

class PopPUNK.models.DBSCANFit(outPrefix, max_samples=100000)

Class for fits using HDBSCAN. Inherits from ClusterFit.

Must first run either fit() or load() before calling other functions

Args:

outPrefix (str)

The output prefix used for reading/writing

max_samples (int)

The number of subsamples to fit the model to

(default = 100000)

assign(X, no_scale=False)

Assign the clustering of new samples using assign_samples_dbscan()

Args:

X (numpy.array)

Core and accessory distances

no_scale (bool)

Do not scale X

[default = False]

Returns:

y (numpy.array)

Cluster assignments by samples

fit(X, max_num_clusters, min_cluster_prop)

Extends fit()

Fits the distances with HDBSCAN and returns assignments by calling fitDbScan().

Fitted parameters are stored in the object.

Args:

X (numpy.array)

The core and accessory distances to cluster. Must be set if preprocess is set.

max_num_clusters (int)

Maximum number of clusters in DBSCAN fitting

min_cluster_prop (float)

Minimum proportion of points in a cluster in DBSCAN fitting

Returns:

y (numpy.array)

Cluster assignments of samples in X

load(fit_npz, fit_obj)

Load the model from disk. Called from loadClusterFit()

Args:

fit_npz (dict)

Fit npz opened with numpy.load()

fit_obj (hdbscan.HDBSCAN)

The saved fit object

plot(X=None, y=None)

Extends plot()

Write a summary of the fit, and plot the results using PopPUNK.plot.plot_dbscan_results()

Args:

X (numpy.array)

Core and accessory distances

y (numpy.array)

Cluster assignments from assign()

save()

Save the model to disk, as an npz and pkl (using outPrefix).

class PopPUNK.models.RefineFit(outPrefix)

Class for fits using a triangular boundary and network properties. Inherits from ClusterFit.

Must first run either fit() or load() before calling other functions

Args:

outPrefix (str)

The output prefix used for reading/writing

apply_threshold(X, threshold)

Applies a boundary threshold, given by user. Does not run optimisation.

Args:

X (numpy.array)

The core and accessory distances to cluster. Must be set if preprocess is set.

threshold (float)

The value along the x-axis (core distance) at which to draw the assignment boundary

Returns:

y (numpy.array)

Cluster assignments of samples in X

assign(X, slope=None)

Assign the clustering of new samples using withinBoundary()

Args:

X (numpy.array)

Core and accessory distances

slope (int)

Override self.slope. Default - use self.slope

Set to 0 for a vertical line, 1 for a horizontal line, or 2 to use a slope

Returns:

y (numpy.array)

Cluster assignments by samples

fit(X, sample_names, model, max_move, min_move, startFile=None, indiv_refine=False, no_local=False, threads=1)

Extends fit()

Fits the distances by optimising network score, by calling refineFit2D().

Fitted parameters are stored in the object.

Args:

X (numpy.array)

The core and accessory distances to cluster. Must be set if preprocess is set.

sample_names (list)

Sample names in X (accessed by iterDistRows())

model (ClusterFit)

The model fit to refine

max_move (float)

Maximum distance to move away from start point

min_move (float)

Minimum distance to move away from start point

startFile (str)

A file defining an initial fit, rather than one from --fit-model. See documentation for format.

(default = None).

indiv_refine (bool)

Run refinement for core and accessory distances separately

(default = False).

no_local (bool)

Turn off the local optimisation step. Quicker, but may be less well refined.

num_processes (int)

Number of threads to use in the global optimisation step.

(default = 1)

Returns:

y (numpy.array)

Cluster assignments of samples in X

load(fit_npz, fit_obj)

Load the model from disk. Called from loadClusterFit()

Args:

fit_npz (dict)

Fit npz opened with numpy.load()

fit_obj (None)

The saved fit object (not used)

plot(X, y=None)

Extends plot()

Write a summary of the fit, and plot the results using PopPUNK.plot.plot_refined_results()

Args:

X (numpy.array)

Core and accessory distances

y (numpy.array)

Assignments (unused)

save()

Save the model to disk, as an npz and pkl (using outPrefix).

PopPUNK.models.loadClusterFit(pkl_file, npz_file, outPrefix='', max_samples=100000)

Call this to load a fitted model

Args:

pkl_file (str)

Location of saved .pkl file on disk

npz_file (str)

Location of saved .npz file on disk

outPrefix (str)

Output prefix for model to save to (e.g. plots)

max_samples (int)

Maximum samples if subsampling X

[default = 100000]

network.py

Functions used to construct the network, and update with new queries. Main entry point is constructNetwork() for new reference databases, and findQueryLinksToNetwork() for querying databases.

Network functions

PopPUNK.network.addQueryToNetwork(dbFuncs, rlist, qfile, G, kmers, assignments, model, queryDB, queryQuery=False, use_mash=False, threads=1)

Finds edges between queries and items in the reference database, and modifies the network to include them.

Args:

dbFuncs (list)

List of backend functions from setupDBFuncs()

rlist (list)

List of reference names

qfile (str)

File containing queries

G (networkx.Graph)

Network to add to (mutated)

kmers (list)

List of k-mer sizes

assignments (numpy.array)

Cluster assignment of items in qlist

model (ClusterModel)

Model fitted to reference database

queryDB (str)

Query database location

queryQuery (bool)

Add in all query-query distances

(default = False)

use_mash (bool)

Use the mash backend

no_stream (bool)

Don’t stream mash output

(default = False)

threads (int)

Number of threads to use if new db created

(default = 1)

Returns:

qlist1 (list)

Ordered list of queries

distMat (numpy.array)

Query-query distances

PopPUNK.network.constructNetwork(rlist, qlist, assignments, within_label, summarise=True)

Construct an unweighted, undirected network without self-loops. Nodes are samples and edges where samples are within the same cluster

Will print summary statistics about the network to STDERR

Args:

rlist (list)

List of reference sequence labels

qlist (list)

List of query sequence labels

assignments (numpy.array)

Labels of most likely cluster assignment from assign_samples()

within_label (int)

The label for the cluster representing within-strain distances from findWithinLabel()

summarise (bool)

Whether to calculate and print network summaries with networkSummary()

(default = True)

Returns:

G (networkx.Graph)

The resulting network

PopPUNK.network.extractReferences(G, mashOrder, outPrefix, existingRefs=None)

Extract references for each cluster based on cliques

Writes chosen references to file by calling writeReferences()

Args:

G (networkx.Graph)

A network used to define clusters from constructNetwork()

mashOrder (list)

The order of files in the sketches, so returned references are in the same order

outPrefix (str)

Prefix for output file (.refs will be appended)

existingRefs (list)

References that should be used for each clique

Returns:

refFileName (str)

The name of the file references were written to

references (list)

An updated list of the reference names

PopPUNK.network.fetchNetwork(network_dir, model, refList, core_only=False, accessory_only=False)

Load the network based on input options

Returns the network as a networkx, and sets the slope parameter of the passed model object.

Args:

network_dir (str)

A network used to define clusters from constructNetwork()

model (ClusterFit)

A fitted model object

refList (list)

Names of references that should be in the network

core_only (bool)

Return the network created using only core distances

[default = False]

accessory_only (bool)

Return the network created using only accessory distances

[default = False]

Returns:

genomeNetwork (nx.Graph)

The loaded network

cluster_file (str)

The CSV of cluster assignments corresponding to this network

PopPUNK.network.networkSummary(G)

Provides summary values about the network

Args:

G (networkx.Graph)

The network of strains from constructNetwork()

Returns:

components (int)

The number of connected components (and clusters)

density (float)

The proportion of possible edges used

transitivity (float)

Network transitivity (triads/triangles)

score (float)

A score of network fit, given by \(\mathrm{transitivity} * (1-\mathrm{density})\)

PopPUNK.network.printClusters(G, outPrefix='_clusters.csv', oldClusterFile=None, externalClusterCSV=None, printRef=True, printCSV=True)

Get cluster assignments

Also writes assignments to a CSV file

Args:

G (networkx.Graph)

Network used to define clusters (from constructNetwork() or addQueryToNetwork())

outPrefix (str)

Prefix for output CSV

Default = “_clusters.csv”

oldClusterFile (str)

CSV with previous cluster assignments. Pass to ensure consistency in cluster assignment name.

Default = None

externalClusterCSV (str)

CSV with cluster assignments from any source. Will print a file relating these to new cluster assignments

Default = None

printRef (bool)

If false, print only query sequences in the output

Default = True

printCSV (bool)

Print results to file

Default = True

Returns:

clustering (dict)

Dictionary of cluster assignments (keys are sequence names)

PopPUNK.network.printExternalClusters(newClusters, extClusterFile, outPrefix, oldNames, printRef=True)

Prints cluster assignments with respect to previously defined clusters or labels.

Args:

newClusters (set iterable)

The components from the graph G, defining the PopPUNK clusters

extClusterFile (str)

A CSV file containing definitions of the external clusters for each sample (does not need to contain all samples)

outPrefix (str)

Prefix for output CSV (_external_clusters.csv)

oldNames (list)

A list of the reference sequences

printRef (bool)

If false, print only query sequences in the output

Default = True

PopPUNK.network.writeDummyReferences(refList, outPrefix)

Writes chosen references to file, for use with mash Gives sequence name twice

Args:

refList (list)

Reference names to write

outPrefix (str)

Prefix for output file (.refs will be appended)

Returns:

refFileName (str)

The name of the file references were written to

PopPUNK.network.writeReferences(refList, outPrefix)

Writes chosen references to file

Args:

refList (list)

Reference names to write

outPrefix (str)

Prefix for output file (.refs will be appended)

Returns:

refFileName (str)

The name of the file references were written to

refine.py

Functions used to refine an existing model. Access using RefineFit.

Refine mixture model using network properties

PopPUNK.refine.decisionBoundary(intercept, gradient)

Returns the co-ordinates where the triangle the decision boundary forms meets the x- and y-axes.

Args:

intercept (numpy.array)

Cartesian co-ordinates of point along line (transformLine()) which intercepts the boundary

gradient (float)

Gradient of the line

Returns:

x (float)

The x-axis intercept

y (float)

The y-axis intercept

PopPUNK.refine.likelihoodBoundary(s, model, start, end, within, between)

Wrapper function around fit2dMultiGaussian() so that it can go into a root-finding function for probabilities between components

Args:

s (float)

Distance along line from mean0

model (BGMMFit)

Fitted mixture model

start (numpy.array)

The co-ordinates of the centre of the within-strain distribution

end (numpy.array)

The co-ordinates of the centre of the between-strain distribution

within (int)

Label of the within-strain distribution

between (int)

Label of the between-strain distribution

Returns:

responsibility (float)

The difference between responsibilities of assignment to the within component and the between assignment

PopPUNK.refine.newNetwork(s, sample_names, distMat, start_point, mean1, gradient, slope=2)

Wrapper function for constructNetwork() which is called by optimisation functions moving a triangular decision boundary.

Given the boundary parameterisation, constructs the network and returns its score, to be minimised.

Args:

s (float)

Distance along line between start_point and mean1 from start_point

sample_names (list)

Sample names corresponding to distMat (accessed by iterator)

distMat (numpy.array)

Core and accessory distances

start_point (numpy.array)

Initial boundary cutoff

mean1 (numpy.array)

Defines line direction from start_point

gradient (float)

Gradient of line to move along

slope (int)

Set to 0 for a vertical line, 1 for a horizontal line, or 2 to use a slope

Returns:

score (float)

-1 * network score. Where network score is from networkSummary()

PopPUNK.refine.readManualStart(startFile)

Reads a file to define a manual start point, rather than using --fit-model

Throws and exits if incorrectly formatted.

Args:

startFile (str)

Name of file with values to read

Returns:

mean0 (numpy.array)

Centre of within-strain distribution

mean1 (numpy.array)

Centre of between-strain distribution

start_s (float)

Distance along line between mean0 and mean1 to start at

PopPUNK.refine.refineFit(distMat, sample_names, start_s, mean0, mean1, max_move, min_move, slope=2, no_local=False, num_processes=1)

Try to refine a fit by maximising a network score based on transitivity and density.

Iteratively move the decision boundary to do this, using starting point from existing model.

Args:

distMat (numpy.array)

n x 2 array of core and accessory distances for n samples

sample_names (list)

List of query sequence labels

start_s (float)

Point along line to start search

mean0 (numpy.array)

Start point to define search line

mean1 (numpy.array)

End point to define search line

max_move (float)

Maximum distance to move away from start point

min_move (float)

Minimum distance to move away from start point

slope (int)

Set to 0 for a vertical line, 1 for a horizontal line, or 2 to use a slope

no_local (bool)

Turn off the local optimisation step. Quicker, but may be less well refined.

num_processes (int)

Number of threads to use in the global optimisation step.

(default = 1)

Returns:

start_point (tuple)

(x, y) co-ordinates of starting point

optimal_x (float)

x-coordinate of refined fit

optimal_y (float)

y-coordinate of refined fit

PopPUNK.refine.transformLine(s, mean0, mean1)

Return x and y co-ordinates for traversing along a line between mean0 and mean1, parameterised by a single scalar distance s from the start point mean0.

Args:

s (float)

Distance along line from mean0

mean0 (numpy.array)

Start position of line (x0, y0)

mean1 (numpy.array)

End position of line (x1, y1)

Returns:

x (float)

The Cartesian x-coordinate

y (float)

The Cartesian y-coordinate

PopPUNK.refine.withinBoundary(dists, x_max, y_max, slope=2)

Classifies points as within or outside of a refined boundary. Numba JIT compiled for speed.

Also used to assign new points in assign()

Args:

dists (numpy.array)

Core and accessory distances to classify

x_max (float)

The x-axis intercept from decisionBoundary()

y_max (float)

The y-axis intercept from decisionBoundary()

slope (int)

Set to 0 for a vertical line, 1 for a horizontal line, or 2 to use a slope

Returns:

signs (numpy.array)

For each sample in dists, -1 if within-strain and 1 if between-strain. 0 if exactly on boundary.

plot.py

Plots of GMM results, k-mer fits, and microreact output

PopPUNK.plot.buildRapidNJ(rapidnj, refList, coreMat, outPrefix, tree_filename)

Use rapidNJ for more rapid tree building

Creates a phylip of core distances, system call to rapidnj executable, loads tree as dendropy object (cleaning quotes in node names), removes temporary files.

Args:

rapidnj (str)

Location of rapidnj executable

refList (list)

Names of sequences in coreMat (same order)

coreMat (numpy.array)

NxN core distance matrix produced in outputsForMicroreact()

outPrefix (int)

Output prefix for temporary files

outPrefix (str)

Prefix for all generated output files, which will be placed in outPrefix subdirectory

tree_filename (str)

Filename for output tree (saved to disk)

Returns:

tree (dendropy.Tree)

NJ tree from core distances

PopPUNK.plot.generate_phylogeny(coreMat, seqLabels, outPrefix, tree_suffix, rapidnj, overwrite)

Generate phylogeny using dendropy or RapidNJ

Writes a neighbour joining tree (.nwk) from core distances.

Args:

coreMat (numpy.array)

n x n array of core distances for n samples.

seqLabels (list)

Processed names of sequences being analysed.

outPrefix (str)

Prefix for all generated output files, which will be placed in outPrefix subdirectory

tree_suffix (str)

String to append to tree file name

rapidnj (str)

A string with the location of the rapidnj executable for tree-building. If None, will use dendropy by default

overwrite (bool)

Overwrite existing output if present (default = False)

PopPUNK.plot.get_grid(minimum, maximum, resolution)

Get a square grid of points to evaluate a function across

Used for plot_scatter() and plot_contours()

Args:

minimum (float)

Minimum value for grid

maximum (float)

Maximum value for grid

resolution (int)

Number of points along each axis

Returns:

xx (numpy.array)

x values across n x n grid

yy (numpy.array)

y values across n x n grid

xy (numpy.array)

n x 2 pairs of x, y values grid is over

PopPUNK.plot.outputsForCytoscape(G, clustering, outPrefix, epiCsv, queryList=None, suffix=None, writeCsv=True)

Write outputs for cytoscape. A graphml of the network, and CSV with metadata

Args:

G (networkx.Graph)

The network to write from constructNetwork()

clustering (dict)

Dictionary of cluster assignments (keys are nodeNames).

outPrefix (str)

Prefix for files to be written

epiCsv (str)

Optional CSV of epi data to paste in the output in addition to the clusters.

queryList (list)

Optional list of isolates that have been added as a query. (default = None)

suffix (string)

String to append to network file name. (default = None)

writeCsv (bool)

Whether to print CSV file to accompany network

PopPUNK.plot.outputsForGrapetree(combined_list, coreMat, clustering, outPrefix, epiCsv, rapidnj, queryList=None, overwrite=False, microreact=False)

Generate files for Grapetree

Write a neighbour joining tree (.nwk) from core distances and cluster assignment (.csv)

Args:

combined_list (list)

Name of sequences being analysed. The part of the name before the first ‘.’ will be shown in the output

coreMat (numpy.array)

n x n array of core distances for n samples.

clustering (dict or dict of dicts)

List of cluster assignments from printClusters(). Further clusterings (e.g. 1D core only) can be included by passing these as a dict.

outPrefix (str)

Prefix for all generated output files, which will be placed in outPrefix subdirectory.

epiCsv (str)

A CSV containing other information, to include with the CSV of clusters

rapidnj (str)

A string with the location of the rapidnj executable for tree-building. If None, will use dendropy by default

queryList (list)

Optional list of isolates that have been added as a query for colouring in the CSV.

(default = None)

overwrite (bool)

Overwrite existing output if present (default = False).

microreact (bool)

Avoid regenerating tree if already built for microreact (default = False).

PopPUNK.plot.outputsForMicroreact(combined_list, coreMat, accMat, clustering, perplexity, outPrefix, epiCsv, rapidnj, queryList=None, overwrite=False)

Generate files for microreact

Output a neighbour joining tree (.nwk) from core distances, a plot of t-SNE clustering of accessory distances (.dot) and cluster assignment (.csv)

Args:

combined_list (list)

Name of sequences being analysed. The part of the name before the first ‘.’ will be shown in the output

coreMat (numpy.array)

n x n array of core distances for n samples.

accMat (numpy.array)

n x n array of accessory distances for n samples.

clustering (dict or dict of dicts)

List of cluster assignments from printClusters(). Further clusterings (e.g. 1D core only) can be included by passing these as a dict.

perplexity (int)

Perplexity parameter passed to t-SNE

outPrefix (str)

Prefix for all generated output files, which will be placed in outPrefix subdirectory

epiCsv (str)

A CSV containing other information, to include with the CSV of clusters

rapidnj (str)

A string with the location of the rapidnj executable for tree-building. If None, will use dendropy by default

queryList (list)

Optional list of isolates that have been added as a query for colouring in the CSV. (default = None)

overwrite (bool)

Overwrite existing output if present (default = False)

PopPUNK.plot.outputsForPhandango(combined_list, coreMat, clustering, outPrefix, epiCsv, rapidnj, queryList=None, overwrite=False, microreact=False)

Generate files for Phandango

Write a neighbour joining tree (.tree) from core distances and cluster assignment (.csv)

Args:

combined_list (list)

Name of sequences being analysed. The part of the name before the first ‘.’ will be shown in the output

coreMat (numpy.array)

n x n array of core distances for n samples.

clustering (dict or dict of dicts)

List of cluster assignments from printClusters(). Further clusterings (e.g. 1D core only) can be included by passing these as a dict.

outPrefix (str)

Prefix for all generated output files, which will be placed in outPrefix subdirectory

epiCsv (str)

A CSV containing other information, to include with the CSV of clusters

rapidnj (str)

A string with the location of the rapidnj executable for tree-building. If None, will use dendropy by default

queryList (list)

Optional list of isolates that have been added as a query for colouring in the CSV. (default = None)

overwrite (bool)

Overwrite existing output if present (default = False)

microreact (bool)

Avoid regenerating tree if already built for microreact (default = False)

PopPUNK.plot.plot_contours(assignments, weights, means, covariances, title, out_prefix)

Draw contours of mixture model assignments

Will draw the decision boundary for between/within in red

Args:

assignments (numpy.array)

n-vectors of cluster assignments for model

weights (numpy.array)

Component weights from BGMMFit

means (numpy.array)

Component means from BGMMFit

covars (numpy.array)

Component covariances from BGMMFit

title (str)

The title to display above the plot

out_prefix (str)

Prefix for output plot file (.pdf will be appended)

PopPUNK.plot.plot_dbscan_results(X, y, n_clusters, out_prefix)

Draw a scatter plot (png) to show the DBSCAN model fit

A scatter plot of core and accessory distances, coloured by component membership. Black is noise

Args:

X (numpy.array)

n x 2 array of core and accessory distances for n samples.

Y (numpy.array)

n x 1 array of cluster assignments for n samples.

n_clusters (int)

Number of clusters used (excluding noise)

out_prefix (str)

Prefix for output file (.png will be appended)

PopPUNK.plot.plot_fit(klist, matching, fit, out_prefix, title)

Draw a scatter plot (pdf) of k-mer sizes vs match probability, and the fit used to assign core and accessory distance

K-mer sizes on x-axis, log(pr(match)) on y - expect a straight line fit with intercept representing accessory distance and slope core distance

Args:

klist (list)

List of k-mer sizes

matching (list)

Proportion of matching k-mers at each klist value

kfit (numpy.array)

Fit to klist and matching from fitKmerCurve()

out_prefix (str)

Prefix for output plot file (.pdf will be appended)

title (str)

The title to display above the plot

PopPUNK.plot.plot_refined_results(X, Y, x_boundary, y_boundary, core_boundary, accessory_boundary, mean0, mean1, start_point, min_move, max_move, scale, threshold, indiv_boundaries, title, out_prefix)

Draw a scatter plot (png) to show the refined model fit

A scatter plot of core and accessory distances, coloured by component membership. The triangular decision boundary is also shown

Args:

X (numpy.array)

n x 2 array of core and accessory distances for n samples.

Y (numpy.array)

n x 1 array of cluster assignments for n samples.

x_boundary (float)

Intercept of boundary with x-axis, from RefineFit

y_boundary (float)

Intercept of boundary with y-axis, from RefineFit

core_boundary (float)

Intercept of 1D (core) boundary with x-axis, from RefineFit

accessory_boundary (float)

Intercept of 1D (core) boundary with y-axis, from RefineFit

mean0 (numpy.array)

Centre of within-strain distribution

mean1 (numpy.array)

Centre of between-strain distribution

start_point (numpy.array)

Start point of optimisation

min_move (float)

Minimum s range

max_move (float)

Maximum s range

scale (numpy.array)

Scaling factor from RefineFit

threshold (bool)

If fit was just from a simple thresholding

indiv_boundaries (bool)

Whether to draw lines for core and accessory refinement

title (str)

The title to display above the plot

out_prefix (str)

Prefix for output plot file (.png will be appended)

PopPUNK.plot.plot_results(X, Y, means, covariances, scale, title, out_prefix)

Draw a scatter plot (png) to show the BGMM model fit

A scatter plot of core and accessory distances, coloured by component membership. Also shown are ellipses for each component (centre: means axes: covariances).

This is based on the example in the sklearn documentation.

Args:

X (numpy.array)

n x 2 array of core and accessory distances for n samples.

Y (numpy.array)

n x 1 array of cluster assignments for n samples.

means (numpy.array)

Component means from BGMMFit

covars (numpy.array)

Component covariances from BGMMFit

scale (numpy.array)

Scaling factor from BGMMFit

out_prefix (str)

Prefix for output plot file (.png will be appended)

title (str)

The title to display above the plot

PopPUNK.plot.plot_scatter(X, scale, out_prefix, title, kde=True)

Draws a 2D scatter plot (png) of the core and accessory distances

Also draws contours of kernel density estimare

Args:

X (numpy.array)

n x 2 array of core and accessory distances for n samples.

scale (numpy.array)

Scaling factor from BGMMFit

out_prefix (str)

Prefix for output plot file (.png will be appended)

title (str)

The title to display above the plot

kde (bool)

Whether to draw kernel density estimate contours

(default = True)

PopPUNK.plot.writeClusterCsv(outfile, nodeNames, nodeLabels, clustering, output_format='microreact', epiCsv=None, queryNames=None)

Print CSV file of clustering and optionally epi data

Writes CSV output of clusters which can be used as input to microreact and cytoscape. Uses pandas to deal with CSV reading and writing nicely.

The epiCsv, if provided, should have the node labels in the first column.

Args:

outfile (str)

File to write the CSV to.

nodeNames (list)

Names of sequences in clustering (includes path).

nodeLabels (list)

Names of sequences to write in CSV (usually has path removed).

clustering (dict or dict of dicts)

Dictionary of cluster assignments (keys are nodeNames). Pass a dict with depth two to include multiple possible clusterings.

output_format (str)

Software for which CSV should be formatted (microreact, phandango, grapetree and cytoscape are accepted)

epiCsv (str)

Optional CSV of epi data to paste in the output in addition to the clusters (default = None).

queryNames (list)

Optional list of isolates that have been added as a query.

(default = None)

sketchlib.py

Sketchlib functions for database construction

PopPUNK.sketchlib.checkSketchlibVersion()

Checks that sketchlib can be run, and returns versiob

Returns:

version (str)

Version string

PopPUNK.sketchlib.constructDatabase(assemblyList, klist, sketch_size, oPrefix, ignoreLengthOutliers=False, threads=1, overwrite=False, reads=False, min_count=0)

Sketch the input assemblies at the requested k-mer lengths

A multithread wrapper around runSketch(). Threads are used to either run multiple sketch processes for each klist value, or increase the threads used by each mash sketch process if len(klist) > threads.

Also calculates random match probability based on length of first genome in assemblyList.

Args:

assemblyList (str)

File with locations of assembly files to be sketched

klist (list)

List of k-mer sizes to sketch

sketch_size (int)

Size of sketch (-s option)

oPrefix (str)

Output prefix for resulting sketch files

ignoreLengthOutliers (bool)

Whether to check for outlying genome lengths (and error if found)

(default = False)

threads (int)

Number of threads to use

(default = 1)

overwrite (bool)

Whether to overwrite sketch DBs, if they already exist.

(default = False)

reads (bool)

If any reads are being used as input, do not run QC

(default = False)

PopPUNK.sketchlib.createDatabaseDir(outPrefix, kmers)

Creates the directory to write sketches to, removing old files if unnecessary

Args:

outPrefix (str)

output db prefix

kmers (list)

k-mer sizes in db

PopPUNK.sketchlib.getKmersFromReferenceDatabase(dbPrefix)

Get kmers lengths from existing database

Args:

dbPrefix (str)

Prefix for sketch DB files

Returns:

kmers (list)

List of k-mer lengths used in database

PopPUNK.sketchlib.getSeqsInDb(dbname)

Return an array with the sequences in the passed database

Args:

dbname (str)

Sketches database filename

Returns:

seqs (list)

List of sequence names in sketch DB

PopPUNK.sketchlib.getSketchSize(dbPrefix)

Determine sketch size, and ensures consistent in whole database

sys.exit(1) is called if DBs have different sketch sizes

Args:

dbprefix (str)

Prefix for mash databases

Returns:

sketchSize (int)

sketch size (64x C++ definition)

PopPUNK.sketchlib.joinDBs(db1, db2, output)

Join two sketch databases with the low-level HDF5 copy interface

Args:

db1 (str)

Prefix for db1

db2 (str)

Prefix for db2

output (str)

Prefix for joined output

PopPUNK.sketchlib.queryDatabase(rNames, qNames, dbPrefix, queryPrefix, klist, self=True, number_plot_fits=0, threads=1)

Calculate core and accessory distances between query sequences and a sketched database

For a reference database, runs the query against itself to find all pairwise core and accessory distances.

Uses the relation \(pr(a, b) = (1-a)(1-c)^k\)

To get the ref and query name for each row of the returned distances, call to the iterator iterDistRows() with the returned refList and queryList

Args:

rNames (list)

Names of references to query

qNames (list)

Names of queries

dbPrefix (str)

Prefix for reference mash sketch database created by constructDatabase()

queryPrefix (str)

Prefix for query mash sketch database created by constructDatabase()

klist (list)

K-mer sizes to use in the calculation

self (bool)

Set true if query = ref

(default = True)

number_plot_fits (int)

If > 0, the number of k-mer length fits to plot (saved as pdfs). Takes random pairs of comparisons and calls plot_fit()

(default = 0)

threads (int)

Number of threads to use in the mash process

(default = 1)

klist (list)

List of k-mer sizes to sketch

Returns:

refList (list)

Names of reference sequences

queryList (list)

Names of query sequences

distMat (numpy.array)

Core distances (column 0) and accessory distances (column 1) between refList and queryList

PopPUNK.sketchlib.readDBParams(dbPrefix, kmers, sketch_sizes)

Get kmers lengths and sketch sizes from existing database

Calls getKmersFromReferenceDatabase() and getSketchSize() Uses passed values if db missing

Args:

dbPrefix (str)

Prefix for sketch DB files

kmers (list)

Kmers to use if db not found

sketch_sizes (list)

Sketch size to use if db not found

Returns:

kmers (list)

List of k-mer lengths used in database

sketch_sizes (list)

List of sketch sizes used in database

PopPUNK.sketchlib.removeFromDB(db_name, out_name, removeSeqs)

Join two sketch databases with the low-level HDF5 copy interface

Args:

db_name (str)

Prefix for hdf database

out_name (str)

Prefix for output (pruned) database

removeSeqs (list)

Names of sequences to remove from database

tsne.py

PopPUNK.tsne.generate_tsne(seqLabels, accMat, perplexity, outPrefix, overwrite, verbosity=0)

Generate t-SNE projection using accessory distances

Writes a plot of t-SNE clustering of accessory distances (.dot)

Args:

seqLabels (list)

Processed names of sequences being analysed.

accMat (numpy.array)

n x n array of accessory distances for n samples.

perplexity (int)

Perplexity parameter passed to t-SNE

outPrefix (str)

Prefix for all generated output files, which will be placed in outPrefix subdirectory

overwrite (bool)

Overwrite existing output if present

(default = False)

verbosity (int)

Verbosity of t-SNE process (0-3)

(default = 0)

utils.py

General utility functions for data read/writing/manipulation in PopPUNK

PopPUNK.utils.assembly_qc(assemblyList, klist, ignoreLengthOutliers)

Calculates random match probability based on means of genomes in assemblyList, and looks for length outliers.

Calls a hard sys.exit(1) if failing!

Args:

assemblyList (str)

File with locations of assembly files to be sketched

klist (list)

List of k-mer sizes to sketch

ignoreLengthOutliers (bool)

Whether to check for outlying genome lengths (and error if found)

Returns:

genome_length (int)

Average length of assemblies

max_prob (float)

Random match probability at minimum k-mer length

PopPUNK.utils.iterDistRows(refSeqs, querySeqs, self=True)

Gets the ref and query ID for each row of the distance matrix

Returns an iterable with ref and query ID pairs by row.

Args:

refSeqs (list)

List of reference sequence names.

querySeqs (list)

List of query sequence names.

self (bool)

Whether a self-comparison, used when constructing a database.

Requires refSeqs == querySeqs

Default is True

Returns:

ref, query (str, str)

Iterable of tuples with ref and query names for each distMat row.

PopPUNK.utils.qcDistMat(distMat, refList, queryList, a_max)

Checks distance matrix for outliers. At the moment just a threshold for accessory distance

Args:

distMat (np.array)

Core and accessory distances

refList (list)

Reference labels

queryList (list)

Query labels (or refList if self)

a_max (float)

Maximum accessory distance to allow

Returns:

passed (bool)

False if any samples failed

PopPUNK.utils.readClusters(clustCSV, return_dict=False)

Read a previous reference clustering from CSV

Args:

clustCSV (str)

File name of CSV with previous cluster assignments

return_type (str)

If True, return a dict with sample->cluster instead of sets

Returns:

clusters (dict)

Dictionary of cluster assignments (keys are cluster names, values are sets containing samples in the cluster). Or if return_dict is set keys are sample names, values are cluster assignments.

PopPUNK.utils.readExternalClusters(clustCSV)

Read a cluster definition from CSV (does not have to be PopPUNK generated clusters). Rows samples, columns clusters.

Args:

clustCSV (str)

File name of CSV with previous cluster assingments

Returns:

extClusters (dict)

Dictionary of dictionaries of cluster assignments (first key cluster assignment name, second key sample, value cluster assignment)

PopPUNK.utils.readPickle(pklName)

Loads core and accessory distances saved by storePickle()

Called during --fit-model

Args:

pklName (str)

Prefix for saved files

Returns:

rlist (list)

List of reference sequence names (for iterDistRows())

qlist (list)

List of query sequence names (for iterDistRows())

self (bool)

Whether an all-vs-all self DB (for iterDistRows())

X (numpy.array)

n x 2 array of core and accessory distances

PopPUNK.utils.readRfile(rFile, oneSeq=False)

Reads in files for sketching. Names and sequence, tab separated

Args:

rFile (str)

File with locations of assembly files to be sketched

oneSeq (bool)

Return only the first sequence listed, rather than a list (used with mash)

Returns:

names (list)

Array of sequence names

sequences (list of lists)

Array of sequence files

PopPUNK.utils.setupDBFuncs(args, kmers, min_count)

Wraps common database access functions from sketchlib and mash, to try and make their API more similar

Args:

args (argparse.opts)

Parsed command lines options

kmers (list)

List of k-mer sizes

min_count (int)

Minimum k-mer count for reads

Returns:

dbFuncs (dict)

Functions with consistent arguments to use as the database API

PopPUNK.utils.storePickle(rlist, qlist, self, X, pklName)

Saves core and accessory distances in a .npy file, names in a .pkl

Called during --create-db

Args:

rlist (list)

List of reference sequence names (for iterDistRows())

qlist (list)

List of query sequence names (for iterDistRows())

self (bool)

Whether an all-vs-all self DB (for iterDistRows())

X (numpy.array)

n x 2 array of core and accessory distances

pklName (str)

Prefix for output files

PopPUNK.utils.translate_distMat(combined_list, core_distMat, acc_distMat)

Convert distances from a square form (2 NxN matrices) to a long form (1 matrix with n_comparisons rows and 2 columns).

Args:

combined_list

Combined list of references followed by queries (list)

core_distMat (numpy.array)

NxN core distances

acc_distMat (numpy.array)

NxN accessory distances

Returns:

distMat (numpy.array)

Distances in long form

PopPUNK.utils.update_distance_matrices(refList, distMat, queryList=None, query_ref_distMat=None, query_query_distMat=None)

Convert distances from long form (1 matrix with n_comparisons rows and 2 columns) to a square form (2 NxN matrices), with merging of query distances if necessary.

Args:

refList (list)

List of references

distMat (numpy.array)

Two column long form list of core and accessory distances for pairwise comparisons between reference db sequences

queryList (list)

List of queries

query_ref_distMat (numpy.array)

Two column long form list of core and accessory distances for pairwise comparisons between queries and reference db sequences

query_query_distMat (numpy.array)

Two column long form list of core and accessory distances for pairwise comparisons between query sequences

Returns:

seqLabels (list)

Combined list of reference and query sequences

coreMat (numpy.array)

NxN array of core distances for N sequences

accMat (numpy.array)

NxN array of accessory distances for N sequences

PopPUNK.utils.writeTmpFile(fileList)

Writes a list to a temporary file. Used for turning variable into mash input.

Args:

fileList (list)

List of files to write to file

Returns:

tmpName (str)

Name of temp file list written to